HPLC chromatography column XBridge™

UHPLCprotein separation

Withstanding extreme operating conditions like high pH and high temperatures is essential for methods development. XBridge HPLC and UHPLC Columns utilize Ethylene Bridged Hybrid (BEH) Particle Technology and are built to withstand high pH, high temperatures, and other extreme operating conditions like volatile eluents, for a wide range of small molecule and biomolecule LC-MS analysis. XBridge HPLC and UHPLC Columns are available in a broad range of sorbent particles and sizes: XBridge eXtended Perfomance (XP) 2.5 µm Columns for rapid analytical UHPLC separations; 3.5 and 5 µm HPLC columns for reliable analytical method development; and 5 and 10 µm optimum bed density (OBD) preparative columns for purification chromatography. Overview Provides high pH stability and chemical resistance for reliability and longevity Enables maximum method development flexibility Available in a range of BEH column chemistries: C18, C8, Shield RP18, Phenyl, HILIC, and Amide fully scalable 2.5 XP, 3.5, 5 μm analytical, and 10 μm OBD preparative particle sizes and column formats Application-specific columns for proteins, peptides, oligonucleotides, and glycan analysis Prolongs column lifetimes with optional XBridge VanGuard Cartridges Recommended Use: For methods development flexibility with improved analyte peak shape and recovery, even under extreme operating conditions. XBridge Column Featured Technologies Ethylene Bridged Hybrid (BEH) Technology Rugged hybrid organic/inorganic base particle Improved peak shape for bases-low silanol activity Wider operating pH range for method development flexibly Mechanical strength for maximum column efficiency at UHPLC operating pressures