Taq DNA polymerase reagent WizPure™

DNA polymerasefor PCRfor DNA sequencing

WizPure™ Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5'→3' direction. Pfu DNA Polymerase also possesses 3'→5'exonuclease (proofreading) activity. WizPure™ Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to tenfold more accurate than normal Taq DNA polymerase. Consequently, WizPure™ Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis. Overview • Error rate : 1.3 x 10-6 • Time to 1kb (72°C) : 60-120 • Thermal stability : Half-life of 4 hrs at 95°C • Blunt end products • Recombinant proteins expressed in E. coli • Free of detectable non-specific nuclease Applications • High fidelity PCR for cloning • Amplification of long DNA fragments (>5-15 kb) • DNA amplification to generate blunt end products • Site-directed mutagenesis • DNA sequencing Storage & Shipping Shipped on ice packs and/or dry ice