Uracil-DNA Glycosylase (UDG) catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.
Applications
• Control of carry-over contamination in PCR
• Site-directed mutagenesis
• As a probe for protein-DNA interaction studies
• SNP genotyping
• Cloning of PCR products
• Generation of single-strand overhangs of PCR products and cDNA
Storage & Shipping
Shipped on ice packs and/or dry ice