Wizol™ Reagent is a complete and ready-to-use reagent for the isolation of total RNA from tissue, cultured animal and bacterial cells, and cell-free fluids such as serum and plasma. The Wizol™ Reagent contains guanidinium, a powerful chaotropic agent effective for rapidly inactivating nucleases, and phenol, an organic solvent used to denature and separate proteins and DNA from RNA.
Wizol™ Reagent ensures total RNA with high yield and good quality from samples of unlimited size. If the larger sample is required, the buffer volume can be scaled proportionately, making this reagent not only very user-friendly but also highly versatile. The total RNA is ready for use in RT-PCR, Northern Blotting, cDNA Synthesis, and Mapping.
Overview
•Ready-to-use
•Room temperature storage
•Simple and fast protocol
Applications
•Total RNA isolation from various sample
•cDNA synthesis
•RT-PCR, Real-time RT-PCR
•Northern/dot blot analysis
•RNase protection assays
•Molecular cloning
Storage & Shipping
Store at room temperature and protect from light
Stable for 12 months if stored under Wizbio's defined conditions