WizPure™ PCR FDmix combines all the reagents necessary for successful routine PCR in a convenient individually aliquot and lyophilized in single-tube.
WizPure™ PCR FDmix is an economical, highly efficient, and ready-to-use PCR premix that can amplify templates of up to 5 kb. The amplification products are compatible with TA cloning.
WizPure™ PCR FDmix yields excellent and consistent results in routine PCR reactions as well as high-throughput PCR genotyping, colony PCR, RT-PCR, and PCR cloning. PCR reactions can be directly loaded onto an agarose gel without the additional need of loading buffer and dyes. WizPure™ PCR FDmix without gel loading dyes is also available for applications when loading dyes are undesired.
Overview
•Including loading dye for easy gel electrophoresis
•A-tail formation at 3’ ends of amplified duplex DNA
•Ready-to-use dried type
•Easy reaction setup (pre-aliquot in 8-strip tube)
•High PCR specificity and high sensitivity PCR amplification
•Room-temperature reaction set-up
•Contains loading dye for visual confirmation and quick electrophoresis
•Minimal optimization requirements
•Eliminating extra pipetting steps and multiple reagents reduces contamination risk and provide better reproducibility
Applications
•High through-put PCR
•Routine diagnostic PCR requiring high reproducibility
•DNA sequencing template preparation