CrystalMix® qPCR-UDG (PROBE) kit is a freeze-dried PCR kit and combines all the reagents necessary for successful routine real-time PCR in a convenient individually aliquot in an 8-strip qPCR tube. CrystalMix® qPCR-UDG (PROBE) kit is an economical, highly efficient, ready-to-use, and room temperature stable format. There is no need for freezing, thawing steps, or pipetting on ice, so minimize the risk of human errors and contaminations.
CrystalMix® qPCR-UDG (PROBE) kit contains antibody-mediated hot-start Taq DNA Polymerase, PCR buffer, MgCl2, and dATP, dCTP, dGTP, dUTP, and Uracil DNA Glycosylase (UDG). UDG and dUTP are included in the mixture to prevent the reamplification of carryover PCR products between reactions. dUTP in the mix ensures that any amplified DNA will contain uracil. UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as a template in future PCRs.
Advantages
Room temperature stable for over 1 year
Ready-to-use freeze-dried type
Fast protocol, just add sample & primer
No freezing/thawing step
No PCR mixture preparation step
No cold chain shipping, Simple transport logistics
Minimize human error and contamination
Maximize Stability, Reproducibility, and Reliability
Aliquot per each reaction
Eliminating extra pipetting steps and reducing contamination risk from multiple reagents and providing better reproducibility
Applications
High through-put Real-time PCR
Gene expression profiling
Gene knockdown verification
Array validation
Routine diagnostic PCR requiring high reproducibility
Point-of-care molecular diagnostics