Giardia is a common intestinal parasite that affects not only dogs but also cats and humans. It can cause symptoms like diarrhea, vomiting, gas, and abdominal discomfort in dogs. The Canine Giardia Antigen AG Test is a lateral flow immunochromatographic assay for the qualitative detection of Giardia antigen (Giardia Ag) in dog's feces or vomit specimen.
PRINCIPLE
The Canine Giardia Ag Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is Giardia antigen in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Giardia antigen in the specimen.
REAGENTS AND MATERIALS
- 10 test pouches, with cards and disposable droppers
- 10 vials of assay buffer (1000μL each)
- 10 Cotton swabs
- 1 package insert
TEST PROCEDURE
- Collect dog's fresh feces or vomit with the cotton swab from dog's anus or from the ground.
- Insert the swab into the provided assay buffer tube. Agitates it to get efficient sample extraction.
- Take out the test device from the foil pouch and place it horizontally.
- Suck the treated sample extraction from the assay buffer tube and place 3 drops into the sample hole "S" of the test device.