Rabies Test Canine Rabies Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of rabies virus antigen (Rabies Ag) in dog's saliva or cerebrospinal fluid.
PRINCIPLE
Rabies Test Canine Rabies Antigen Rapid Test was based on sandwich lateral flow immunochromatography. The test device has a test window for observation analysis run and result reading. The test window has an invisible T( test) area and a C( control) area.
When the treated sample is coated into the sample hole on the equipment, the liquid will flow horizontally through the surface of the test strip and react with the pre-coated monoclonal antibody. if rabies antigen is present in the specimen, a visible T line appears.
C rows should always appear after the application example, which represents a valid result. By this method, the device can accurately indicate the presence of rabies virus antigen in the specimen.
REAGENTS AND MATERIALS
- 10 test pouches, with cards and disposable droppers
- 10 vials of assay buffer (1000μL each)
- 10 bags of cotton swab stick
- 1 package insert
TEST PROCEDURE
- Collect the dog's saliva with a cotton swab and thoroughly moisten the swab.
If an autopsy can be performed, the cerebrospinal fluid can be measured with a cotton swab.
- Insert the wet cotton swab into the connected measuring buffer tube.
Stir to obtain an effective sample extraction.
- Remove the test equipment from the foil bag and place it horizontally.
- Extract the treated sample extract from the test buffer tube and drop 3 drops into the sample hole "S" of the test equipment.