Solution reagent Hieff NGS™

magnetic bead-basedNGSfor DNA purification

Hieff NGS™ DNA Selection Beads are prepared based on the SPRI (Solid Phase Reverse Immobilization) principle and is applicable for DNA purification and size selection during the preparation of next generation sequencing (NGS) libraries. Hieff NGS™ DNA Selection Beads is compatible with various of DNA and RNA library prep kits. Specifications Product Line - DNA clean and selection Beads Starting Material - DNA Compatibility - DNA Isolation Technology - Magnetic Bead Final Product Type - DNA For Use With (Application) - DNA celan up, DNA size slection Instructions 1. Preparation Equilibrate the selection beads at room temperature for at least 30 min before use. 2.1Mix the beads thoroughly by vortexing or pipetting up and down every time before using. 2.2Add the first round of selection beads to the sample (refer to Table 1). Mix thoroughly by vortexing or pipetting up and down at least 10 times. 2.3Incubate at room temperature for 5 min. 2.4Spin down the tube briefly and place it on magnetic stand. When the solution is clear (about 5 min), transfer the supernatant to a new PCR tube. 2.5Add the second round of selection beads to the sample from step 2.4 according to Table 1. Mix thoroughly by vortexing or pipetting up and down at least 10 times. 2.6Incubate at room temperature for 5 min. 2.7Spin down the tube briefly and place it on magnetic stand. When the solution is clear (about 5 min), aspirate the supernatant and discard. 2.8Keep the tube in the magnetic stand and add 200 μL of freshly prepared 80% ethanol to without disturbing the beads, incubate at room temperature for 30 sec. Aspirate the ethanol and discard. fly and