2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly for electrophoresis with pre-loaded bromophenol blue dye. The amplified products contain 3 '-dA protrusion and can be easily cloned into T vector. The 2×HieffTM PCR Master Mix simplifies PCR procedure and reduces contamination.
Features
Convenient, ready-to-use mix
Thermostable—half-life is more than 40 min at 95°C
Generates PCR products with 3'-dA overhangs
Applications
Routine PCR amplification of DNA fragments
High throughput PCR
DNA labeling
Specifications
Fidelity (vs. Taq) - 1 ×
Hot Start - No
Overhang - 3'-A
Polymerase - Taq DNA Polymerase
Reaction Format - SuperMix or Master Mix
Reaction Speed - Standard
Product Type - PCR Master Mix (2×)