Enzyme reagent 10298ES series

DNA ligasefor researchNGS

Hieff™ Novel T4 DNA Ligase is a single enzyme product that can be used for DNA fragments and adapters ligation during NGS library preparation. The product has been verified by high-throughput sequencing and has excellent quality. This enzyme has efficient ligation ability and is compatible with various samples. Unit Definition The amount of enzyme required to catalyze the ligation of more than 50% of the DNA fragments in a 20 μL ligation reaction system is defined as 1 activity unit (U ) when 6 μg of λDNA Hind III catabolite is reacted at 16 °C for 30 min. Cautions 1. For your safety and health, please wear lab coats and disposable gloves for operation. 2. For research use only! Component Volume (μL) dA-tailed DNA 60 10 × T4 DNA Ligase Buffer 10 50% PEG 6000 10* DNA Adapter x Hieff™ Novel T4 DNA Ligase 1-5 ddH2O To 100