1×dsDNA HS Assay Kit is a rapid, highly sensitive and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). This kit is highly selective for dsDNA and has good linearity in the range of 0.2 ng-100 ng, the quantitation range is between 10 pg/μL to 100 ng/μL. This kit is easy to operate, providing a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometer or Fluorescence Microplate Reader. It is ideal choice for NGS large-scale DNA sample quantification (such as input DNA quantification, DNA library quantification, etc.). This kit is well tolerated to common contaminants such as proteins and salts.
Features
High sensitive: the concentrations ranging from 10 pg/µ l to 100 ng/µL of dsDNA can be accurately quantified;
High specificity: this product is highly selective to dsDNA and is not affected by RNA, has good tolerance to some conventional pollutants, such as salt, free nucleotide, protein, solvent, detergent, etc;
Ready to use: 1x working solution, just add the dsDNA sample to be tested and test it with Qubit fluorometer or Fluorescence Microplate Reader at room temperature
Applications
dsDNA quantification with Qubit or Microplate Reader
NGS library quantification
Specifications
Excitation/Emission - 480/520
For Use With (Equipment) - Qubit Fluorometer, Microplate Reader
No. of Reactions - 100T/500T
Product Line - Qubit Quantitation
Quantitation Range - 10 pg/μL to 100 ng/μL
Sample Volume - 1 μL to 20 μL
Detection Method - Fluorescent