Solution reagent kit Hieff NGSTM

for RNA extractionNGS

Hieff NGSTM MaxUp Human rRNA Depletion Kit (rRNA & ITS/ETS) is designed to remove rRNA and 45S ITS/ETS from human, mouse and rat total RNA based on RNase H-based workflow and to retain mRNA and other non-coding RNA. This kit is suitable for both intact and partially degraded RNA samples (e.g., FFPE RNA). Since degraded FFPE samples usually contain a higher proportion of ITS/ETS than fresh tissue samples, this kit adds probes in the Human 45S ITS/ETS region, and ITS removal can significantly increase the proportion of valid data in raw data. Features Strong specificity: specifically remove rRNA and ITS/ETS from human samples, especially for FFPE samples High compatibility of template starting amount: applicable to 100 ng~1μg sample High removal effect: for rRNA, ITS and ETS in human\mouse and rat samples, the removal effect is more than 95% Stable quality: strict batch performance and stability quality control Applications Gene Expression Research Alternative splicing analysis Detection and discovery of non coding RNA dentification of selective polyadenylation sites Fusion gene detection Specifications Depletion Technology - RNase H Sample Type - Total RNA of human, mouse and rat Final Product Type - mRNA and other non coding RNAs No. of Reactions - 24/96 Preps Starting Material Amount - 100 ng~1 μg total RNA Target - Remove rRNA and 45S ITS/ETS form human total RNA Figures rRNA removal specificity For conventional samples, rRNA and ITS/EST can be removed by more than 98%, while for FFPE samples with integrity of about 50%, rRNA and ITS/EST can be removed by more than 95%. At the same time, FFPE with poor integrity can also be removed effectively.