Bst DNA polymerase reagent Hieff™

solutionenzymefor molecular biology

Hieff™ Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. The enzyme has stronger 5'-3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc. Features Thermophilic DNA polymerase with strong strand displacement activity Applications Isothermal DNA amplification Specifications Polymerase - Bst DNA Polymerase Heat Inactivation - Incubation at 85°C for 5 min. Solution Components - 10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃. Activity Definition - 1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.