Hieff™ Taq DNA polymerase is a thermostable recombinant DNA polymerase expressed by Thermus aquaticus with a molecular weight of 94 KDa. It has 5'→3' polymerase activity and 5'→3' exonuclease activity but without 3'→5' exonuclease activity. The amplified product has 3'- dA and can be directly used for TA cloning.
Features
5'→3' DNA polymerase activity
5'→3' exonuclease activity
Hieff™ Taq DNA polymerase is an ideal tool for standard PCR with templates of 5 kb or less
Applications
Genotyping, colony PCR and other conventional PCR
Specifications
Polymerase - Taq DNA polymerase
Molecular Weight - 94 KDa
Unit Definition - Using the activated DNA of salmon sperm as template/primer, the activity is defined as one active unit (U) when 10 nmol of total nucleotide was ingested as acid insoluble substance at 74℃ for 30 min.