This product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA.
Note: G* is the first base of the RNA transcript.
This product is produced in accordance with GMP regulations and provided in liquid form.
Feature
Synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template
Higher yield and easy to operate
Lower endotoxins
Tested for the absence of endonucleases, exonucleases, RNases
Application
Radiolabeled RNA probe preparation
RNA generation for studies of RNA structure, processing and catalysis
Expression control via anti-sense RNA
Specification
Source - Recombinant E. coli with T7 RNA Polymerase gene
Optimum Temperature - 37℃
Storage Buffer - 50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100,50% (v/v) glycerin,pH7.9 at 25℃
Unit Definition - The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.