RNA Synthesis reagent kit 10623ES series

dNTP solutionbuffer solutionliquid

T7 High Yield RNA Synthesis Kit optimizes the transcription reaction system. The kit can synthesize the single-stranded RNA efficiently by using T7 RNA polymerase, the linear double-stranded DNA with the T7 promoter sequence as the template, NTPs as the substrate to control the DNA sequence downstream of the promoter. During transcription, modified nucleotides can be added to the substrate to prepare biotin or dye-labeled RNA. This kit can synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template input. The RNA synthesized by transcription can be used for various downstream applications, such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation. Feature Up to 180 μg of RNA per reaction from 1 μg of the control template Optimized reaction system for the IVT process Decrease the dsRNA production Higher RNA integrity and purity FAQs: 1. Low transcript yield The quality of the template is closely related to the yield. If the yield of the experimental group is significantly lower than the control group, the possible reasons are: ① the experimental template contains inhibitory components; ② The template has something wrong. Suggestions: ① Re-purify the template; ② Determine the template quantification and its integrity; ③ Extend the reaction time; ④ Increase the amount of template input; ⑤ Try other promoters and RNA polymerases.