Highest Yield: Robust cfDNA isolation from up to 10 mL plasma w/ unique MAGicBead™ surface technology
NGS-Ready: High-quality cfDNA from plasma, saliva, urine, etc. is ideal for NGS
Compatible: With all open automation platforms
DESCRIPTION
The MAGicBead™ cfDNA Isolation Kit features a unique, magnetic bead surface chemistry that facilitates unparalleled cfDNA recovery from biofluids. Input volumes can range up to 10 mL of plasma. It is compatible with all open automation platforms and a wide range of sample types (serum, saliva, urine, etc.). This kit features a simple, streamlined extraction process with minimal number of steps and does not require the bead drying step common with other similar procedures. Eluted cfDNA is free of enzymatic inhibitors that can interfere with PCR or other sensitive downstream applications, including Next-Gen Sequencing.
Specifications
Elution Volume - ≥ 15 µl MAGicBead™ cfDNA Elution Buffer.
Fragment Sizes - For plasma samples, DNA fragments will typically display a mono-nucleosomal peak in the 140-170 bp range and a genomic DNA fragments peak > 10 kb. However, other multi-nucleosomal apoptotic DNA fragments may also be present.
Processing Time - 1-1.5 hour (~15 minutes hands-on time).
Purity - Eluted cfDNA is NGS-ready. NOTE: Due to low levels of cfDNA available in cell-free biofluids, a spectrophotometer (e.g., NanoDrop) is not recommended for quantification or purity assessment.
Required Equipment - An open automation platform: KingFisher Flex/Apex (Thermo-Fisher Scientific), Fluent® X (Tecan), Microlab® STAR™ (Hamilton), OT-2 (Opentrons) and others.