Enzyme reagent E101 series

for microbiologyfor nucleic acidslyophilized

DNase I (RNase-free) cuts both double-stranded and single-stranded DNA, producing 3′-OH oligonucleotides. It is typically used for selectively degrading DNA in the presence of RNA. This DNase is suited for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR. It is compatible with all of our RNA kits featuring in-column DNase digestion. The DNase I Set features lyophilized enzyme and DNA Digestion Buffer. TECHNICAL SPECIFICATIONS Concentration1 U/µl Heat inactivationAdd a final concentration of 5 mM EDTA, then incubate at 65°C for 10 min.. IncludedOne unit (U) is defined as the amount of enzyme required to degrade 1 µg λ DNA completely in 10 minutes at 37°C in a 50 µl reaction volume (40 mM Tris-HCl, pH 8.0, 10 mM NaCl, 6 mM MgCl2, and 10 mM CaCl2). One unit of enzyme is equivalent to one Kunitz unit. Resuspension Volume275 µl Size250 U