Solution reagent kit D503 series

for PCRfor next-generation sequencingfor bisulfite conversion

HIGHLIGHTS Fastest 96-well bisulfite conversion kit for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis. Ready-to-use conversion reagent is added directly to DNA. High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc. DESCRIPTION The EZ-96 DNA Methylation-Lightning Kit is a rapid, high-throughput (96-well) bisulfite conversion kit for DNA methylation analysis. The streamlined workflow features ready-to-use Lightning Conversion Reagent and a combined DNA denaturation and bisulfite conversion process. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. With the Deep-Well Kit (Cat. D5033), samples can be eluted in as little as 15 µl. High yield, converted DNA is ideal for PCR, array, and Next-Generation sequencing, etc. TECHNICAL SPECIFICATIONS ApplicationsPurified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc. Conversion> 99.5% Elution VolumeDeep-well: ≥ 15 µl Shallow-well: ≥ 30 µl EquipmentThermocycler with heated lid, swinging-bucket centrifuge with plate carriers. Input100 pg - 2 µg of DNA. Processing Time1.5 hours Recovery> 80% Sample SourcePurified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.