Solution reagent kit R1017

for molecular biologyRT-qPCRfor nucleic acids

NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc. Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes. Maximum Recovery: Recover >90% and elute in as little as 25 μl. DESCRIPTION The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 25 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations. TECHNICAL SPECIFICATIONS EquipmentMicrocentrifuge FormatSpin-Column PurityRNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. Sample SourceDNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction Size RangeTotal RNA ≥ 17 nt Yield50 µg RNA (binding capacity), ≥ 25 µl (elution volume)