LiNEAR chromogenic reagents

Storage temperature: 2 °C - 8 °C

The method is based on the hydrolysis of α-naphtyl phosphate at pH 5.0 by acid phosphatase (ACP) to produce α-naphtol and inorganic phosphate. The pentanediol acts as a phosphate acceptor increasing the reaction sensitivity. The α-naphtol ...

Storage temperature: 2 °C - 8 °C

The method is based on the specific binding of bromocresol green (BCG), an anionic dye, and the protein at acid pH with the resulting shift in the absorption wavelength of the complex. The intensity of the color formed is proportional ...

Storage temperature: 2 °C - 8 °C

Alkaline phosphatase (ALP) catalyze the hydrolysis of 4-nitrophenylphosphate (4-NPP) with the formation of free 4-nitrophenol and inorganic phosphate, acting the alkaline buffer as a phosphate-group acceptor. The reaction is monitored ...

Storage temperature: 2 °C - 8 °C

In this direct method α-amylase catalyzes the hydrolysis of 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNP-G3) substrate at pH 6.0 forming 2-chloro-p-nitrophenol (CNP) and free glycosides. The reaction is monitored kinetically at 405 ...

Storage temperature: 2 °C - 8 °C

Bilirubin is converted to coloured azobilirubin by diazotized sulfanilic acid and is measured photometrically. Of the two bilirubin fractions in serum –bilirubin-glucuronide and free bilirubin which is bound to albumin– only the former ...

Storage temperature: 2 °C - 8 °C

Direct bilirubin (conjugated) reacts with the diazonium salt 2,4-dichlorophenyldiazonium (2,4-DPD) in the presence of sulfamic acid to form azo-bilirubin, this coloured complex can be measured photometrically at 546 nm. Of the two bilirubin ...

Storage temperature: 2 °C - 8 °C

The method is based on the specific binding of arsenazo III and calcium at acid pH with the resulting shift in the absorbtion wavelength of the complex. The intensity of the cromophore formed is proportional to the concentration of total ...

Storage temperature: 2 °C - 8 °C

Chloride ions in the sample quantitatively displaces thiocyanate from mercuric thiocyanate. Liberated thiocyanate ion reacts with ferric ion forming a red ferric-thiocyanate complex proportional to the concentration of chloride present in the sample.

Storage temperature: 2 °C - 8 °C

This method for the measurement of total cholesterol in serum involves the use of three enzymes: cholesterol esterase (CE), cholesterol oxidase (CO) and peroxidase (POD). In the presence of the former the mixture of phenol and 4-aminoantipyrine ...

Storage temperature: 2 °C - 8 °C

Cholinesterase (CHE) catalyzes the hydrolysis of butyrylthiocholine substrate forming butyrate and thiocholine. The latter reduces 5,5´- mercaptobis-2-nitrobenzoic acid (DMNB) to 5-mercapto-2- nitrobenzoate (5-MNBA), a colored compound. The ...