LiNEAR enzymatic reagents

Alanine aminotransferase (ALT/GPT) catalyzes the transfer of the amino group from alanine to oxoglutarate with the formation of glutamate and pyruvate. The latter is reduced to lactate by lactate dehydrogenase (LDH) in the presence of ...

Storage temperature: 2 °C - 8 °C

In this direct method α-amylase catalyzes the hydrolysis of 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNP-G3) substrate at pH 6.0 forming 2-chloro-p-nitrophenol (CNP) and free glycosides. The reaction is monitored kinetically at 405 ...

Storage temperature: 2 °C - 8 °C

Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to oxoglutarate with the formation of glutamate and oxalacetate. The latter is reduced to malate by malate dehydrogenase (MDH) in the presence ...

Storage temperature: 2 °C - 8 °C

This method for the measurement of total cholesterol in serum involves the use of three enzymes: cholesterol esterase (CE), cholesterol oxidase (CO) and peroxidase (POD). In the presence of the former the mixture of phenol and 4-aminoantipyrine ...

Storage temperature: 2 °C - 8 °C

Cholinesterase (CHE) catalyzes the hydrolysis of butyrylthiocholine substrate forming butyrate and thiocholine. The latter reduces 5,5´- mercaptobis-2-nitrobenzoic acid (DMNB) to 5-mercapto-2- nitrobenzoate (5-MNBA), a colored compound. The ...

Storage temperature: 2 °C - 8 °C

Creatine kinase (CK) catalyzes the reaction between creatine phosphate (CP) and adenosine 5´-diphosphate (ADP) with formation of creatine and adenosine 5´-triphosphate (ATP). The latter phosphorylates glucose to glucose-6-phosphate (G6P) ...

Storage temperature: 2 °C - 8 °C

This procedure is based upon an improved enzymatic method originally devised to evaluate creatinine in serum and urine. The test is performed in two steps. In the first step creatine is removed during the first minutes ...

Storage temperature: 2 °C - 8 °C

... -nitro-benzoate. The amount of 5-amino-2-nitro-benzoate formed, monitored kinetically at 405 nm, is proportional to the enzyme activity present in the sample.

Storage temperature: 2 °C - 8 °C

In the Trinder reaction, the glucose is oxidized to D-gluconate by the glucose oxidase (GOD) with the formation of hydrogen peroxide. In the presence of peroxidase (POD), a mixture of phenol and 4-aminoantipyrine (4-AA) is oxidized by ...

Storage temperature: 2 °C - 8 °C

... VLDL, LDL and (a)Lpa) by phosphotungstic acid/MgCl2, sedimentation of the precipitant by centrifugation, and subsequent enzymatic analysis of high density lipoproteins (HDL) as residual cholesterol remaining in the clear ...