LiNEAR enzyme reagents

Alanine aminotransferase (ALT/GPT) catalyzes the transfer of the amino group from alanine to oxoglutarate with the formation of glutamate and pyruvate. The latter is reduced to lactate by lactate dehydrogenase (LDH) in the presence of ...

Storage temperature: 2 °C - 8 °C

The method is based on the hydrolysis of α-naphtyl phosphate at pH 5.0 by acid phosphatase (ACP) to produce α-naphtol and inorganic phosphate. The pentanediol acts as a phosphate acceptor increasing the reaction sensitivity. The α-naphtol ...

Storage temperature: 2 °C - 8 °C

Alkaline phosphatase (ALP) catalyze the hydrolysis of 4-nitrophenylphosphate (4-NPP) with the formation of free 4-nitrophenol and inorganic phosphate, acting the alkaline buffer as a phosphate-group acceptor. The reaction is monitored ...

Storage temperature: 2 °C - 8 °C

In this direct method α-amylase catalyzes the hydrolysis of 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNP-G3) substrate at pH 6.0 forming 2-chloro-p-nitrophenol (CNP) and free glycosides. The reaction is monitored kinetically at 405 ...

Storage temperature: 2 °C - 8 °C

Direct bilirubin (conjugated) reacts with the diazonium salt 2,4-dichlorophenyldiazonium (2,4-DPD) in the presence of sulfamic acid to form azo-bilirubin, this coloured complex can be measured photometrically at 546 nm. Of the two bilirubin ...

Storage temperature: 2 °C - 8 °C

Cholinesterase (CHE) catalyzes the hydrolysis of butyrylthiocholine substrate forming butyrate and thiocholine. The latter reduces 5,5´- mercaptobis-2-nitrobenzoic acid (DMNB) to 5-mercapto-2- nitrobenzoate (5-MNBA), a colored compound. The ...

Storage temperature: 2 °C - 8 °C

... activity of CK present in the sample. In this test the presence of N-acetilcysteine (NAC) allows the optimal activation of the enzyme.

Storage temperature: 2 °C - 8 °C

... mainly in skeletal and heart muscle. CK-BB is ussualy present in serum at low concentration. Both, creatine kinase (CK) enzymes are dimers formed by the association of two sub-units from muscle (M) and nerve cells ...

Storage temperature: 2 °C - 8 °C

... -nitro-benzoate. The amount of 5-amino-2-nitro-benzoate formed, monitored kinetically at 405 nm, is proportional to the enzyme activity present in the sample.

Storage temperature: 2 °C - 8 °C

Aspartate aminotransferase (GOT) catalyzes the transfer of the amino group from aspartate to oxoglutarate with the formation of glutamate and oxalacetate. L-Aspartate + 2-Oxoglutarate L-Glutamate + Oxalacetate Alanine aminotransferase ...