LiNEAR reagents

Storage temperature: 2 °C - 8 °C

The method is based on the hydrolysis of α-naphtyl phosphate at pH 5.0 by acid phosphatase (ACP) to produce α-naphtol and inorganic phosphate. The pentanediol acts as a phosphate acceptor increasing the reaction sensitivity. The α-naphtol ...

Storage temperature: 2 °C - 8 °C

The method is based on the specific binding of bromocresol green (BCG), an anionic dye, and the protein at acid pH with the resulting shift in the absorption wavelength of the complex. The intensity of the color formed is proportional ...

Storage temperature: 2 °C - 8 °C

Alkaline phosphatase (ALP) catalyze the hydrolysis of 4-nitrophenylphosphate (4-NPP) with the formation of free 4-nitrophenol and inorganic phosphate, acting the alkaline buffer as a phosphate-group acceptor. The reaction is monitored ...

Alanine aminotransferase (ALT/GPT) catalyzes the transfer of the amino group from alanine to oxoglutarate with the formation of glutamate and pyruvate. The latter is reduced to lactate by lactate dehydrogenase (LDH) in the presence of ...

Storage temperature: 2 °C - 8 °C

In this direct method α-amylase catalyzes the hydrolysis of 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNP-G3) substrate at pH 6.0 forming 2-chloro-p-nitrophenol (CNP) and free glycosides. The reaction is monitored kinetically at 405 ...

Storage temperature: 2 °C - 8 °C

Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to oxoglutarate with the formation of glutamate and oxalacetate. The latter is reduced to malate by malate dehydrogenase (MDH) in the presence ...

Storage temperature: 2 °C - 8 °C

Bilirubin is converted to coloured azobilirubin by diazotized sulfanilic acid and is measured photometrically. Of the two bilirubin fractions in serum –bilirubin-glucuronide and free bilirubin which is bound to albumin– only the former ...

Storage temperature: 2 °C - 8 °C

Direct bilirubin (conjugated) reacts with the diazonium salt 2,4-dichlorophenyldiazonium (2,4-DPD) in the presence of sulfamic acid to form azo-bilirubin, this coloured complex can be measured photometrically at 546 nm. Of the two bilirubin ...

Storage temperature: 2 °C - 8 °C

The method is based on the specific binding of arsenazo III and calcium at acid pH with the resulting shift in the absorbtion wavelength of the complex. The intensity of the cromophore formed is proportional to the concentration of total ...

Storage temperature: 2 °C - 8 °C

The method is based on the specific binding of cresolftalein complexone (OCC), a metallochromic indicator, and calcium at alkaline pH with the resulting shift in the absorbtion wavelength of the complex. The intensity of the cromophore ...