LiNEAR solution reagents

Storage temperature: 2 °C - 8 °C

Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to oxoglutarate with the formation of glutamate and oxalacetate. The latter is reduced to malate by malate dehydrogenase (MDH) in the presence ...

Storage temperature: 2 °C - 8 °C

Bilirubin is converted to coloured azobilirubin by diazotized sulfanilic acid and is measured photometrically. Of the two bilirubin fractions in serum –bilirubin-glucuronide and free bilirubin which is bound to albumin– only the former ...

Storage temperature: 2 °C - 8 °C

Direct bilirubin (conjugated) reacts with the diazonium salt 2,4-dichlorophenyldiazonium (2,4-DPD) in the presence of sulfamic acid to form azo-bilirubin, this coloured complex can be measured photometrically at 546 nm. Of the two bilirubin ...

Storage temperature: 2 °C - 8 °C

The method is based on the specific binding of arsenazo III and calcium at acid pH with the resulting shift in the absorbtion wavelength of the complex. The intensity of the cromophore formed is proportional to the concentration of total ...

Storage temperature: 2 °C - 8 °C

Chloride ions in the sample quantitatively displaces thiocyanate from mercuric thiocyanate. Liberated thiocyanate ion reacts with ferric ion forming a red ferric-thiocyanate complex proportional to the concentration of chloride present in the sample.

Storage temperature: 2 °C - 8 °C

This method for the measurement of total cholesterol in serum involves the use of three enzymes: cholesterol esterase (CE), cholesterol oxidase (CO) and peroxidase (POD). In the presence of the former the mixture of phenol and 4-aminoantipyrine ...

Storage temperature: 2 °C - 8 °C

Cholinesterase (CHE) catalyzes the hydrolysis of butyrylthiocholine substrate forming butyrate and thiocholine. The latter reduces 5,5´- mercaptobis-2-nitrobenzoic acid (DMNB) to 5-mercapto-2- nitrobenzoate (5-MNBA), a colored compound. The ...

Storage temperature: 2 °C - 8 °C

Creatine kinase (CK) catalyzes the reaction between creatine phosphate (CP) and adenosine 5´-diphosphate (ADP) with formation of creatine and adenosine 5´-triphosphate (ATP). The latter phosphorylates glucose to glucose-6-phosphate (G6P) ...

Storage temperature: 2 °C - 8 °C

The major CK activity in normal serum is due to CK-MM and CKMB isoenzymes, found mainly in skeletal and heart muscle. CK-BB is ussualy present in serum at low concentration. Both, creatine kinase (CK) enzymes are dimers formed by the ...

Storage temperature: 2 °C - 8 °C

This procedure is based upon a modification of the original picrate reaction (Jaffe). Creatinine under alkaline conditions reacts with picrate ions forming a reddish complex. The formation rate of the complex measured through the increase ...